A histone H4 promoter for expression of a phleomycin-resistance gene in Phanerochaete chrysosporium.
Identifieur interne : 000D72 ( Main/Exploration ); précédent : 000D71; suivant : 000D73A histone H4 promoter for expression of a phleomycin-resistance gene in Phanerochaete chrysosporium.
Auteurs : M. Gessner [Allemagne] ; U. RaederSource :
- Gene [ 0378-1119 ] ; 1994.
Descripteurs français
- KwdFr :
- ADN fongique (métabolisme), Basidiomycota (croissance et développement), Basidiomycota (effets des médicaments et des substances chimiques), Basidiomycota (génétique), Histone (génétique), Marqueurs génétiques (génétique), Méthylation (MeSH), Phléomycines (pharmacologie), Protéines bactériennes (génétique), Régions promotrices (génétique) (génétique), Résistance microbienne aux médicaments (génétique), Transformation génétique (MeSH), Vecteurs génétiques (MeSH).
- MESH :
- croissance et développement : Basidiomycota.
- effets des médicaments et des substances chimiques : Basidiomycota.
- génétique : Basidiomycota, Histone, Marqueurs génétiques, Protéines bactériennes, Régions promotrices (génétique), Résistance microbienne aux médicaments.
- métabolisme : ADN fongique.
- pharmacologie : Phléomycines.
- Méthylation, Transformation génétique, Vecteurs génétiques.
English descriptors
- KwdEn :
- Bacterial Proteins (genetics), Basidiomycota (drug effects), Basidiomycota (genetics), Basidiomycota (growth & development), DNA, Fungal (metabolism), Drug Resistance, Microbial (genetics), Genetic Markers (genetics), Genetic Vectors (MeSH), Histones (genetics), Methylation (MeSH), Phleomycins (pharmacology), Promoter Regions, Genetic (genetics), Transformation, Genetic (MeSH).
- MESH :
- chemical , genetics : Bacterial Proteins, Genetic Markers, Histones.
- drug effects : Basidiomycota.
- genetics : Basidiomycota, Drug Resistance, Microbial, Promoter Regions, Genetic.
- growth & development : Basidiomycota.
- chemical , metabolism : DNA, Fungal.
- chemical , pharmacology : Phleomycins.
- Genetic Vectors, Methylation, Transformation, Genetic.
Abstract
In this study, two transformation vectors (pMG101 and pMG103) for Phanerochaete chrysosporium were constructed, based on the ble phleomycin-resistance-encoding gene and a homologous histone H4 promoter. Transformation frequencies were 6-10 per micrograms of DNA. Transformed vector DNA could either exist as an unstable replicating plasmid or could be stably integrated. Integrated vector DNA from pMG101, which also contains a histone-encoding H3 gene in the promoter fragment, becomes methylated, resulting in inactivation of ble-dependent resistance. Plasmid pMG103, which lacks the H3, does not show methylation.
DOI: 10.1016/0378-1119(94)90267-4
PubMed: 8194757
Affiliations:
Links toward previous steps (curation, corpus...)
Le document en format XML
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Transformation frequencies were 6-10 per micrograms of DNA. Transformed vector DNA could either exist as an unstable replicating plasmid or could be stably integrated. Integrated vector DNA from pMG101, which also contains a histone-encoding H3 gene in the promoter fragment, becomes methylated, resulting in inactivation of ble-dependent resistance. Plasmid pMG103, which lacks the H3, does not show methylation.</div></front></TEI><pubmed><MedlineCitation Status="MEDLINE" Owner="NLM"><PMID Version="1">8194757</PMID><DateCompleted><Year>1994</Year><Month>06</Month><Day>29</Day></DateCompleted><DateRevised><Year>2019</Year><Month>07</Month><Day>07</Day></DateRevised><Article PubModel="Print"><Journal><ISSN IssnType="Print">0378-1119</ISSN><JournalIssue CitedMedium="Print"><Volume>142</Volume><Issue>2</Issue><PubDate><Year>1994</Year><Month>May</Month><Day>16</Day></PubDate></JournalIssue><Title>Gene</Title><ISOAbbreviation>Gene</ISOAbbreviation></Journal><ArticleTitle>A histone H4 promoter for expression of a phleomycin-resistance gene in Phanerochaete chrysosporium.</ArticleTitle><Pagination><MedlinePgn>237-41</MedlinePgn></Pagination><Abstract><AbstractText>In this study, two transformation vectors (pMG101 and pMG103) for Phanerochaete chrysosporium were constructed, based on the ble phleomycin-resistance-encoding gene and a homologous histone H4 promoter. Transformation frequencies were 6-10 per micrograms of DNA. Transformed vector DNA could either exist as an unstable replicating plasmid or could be stably integrated. Integrated vector DNA from pMG101, which also contains a histone-encoding H3 gene in the promoter fragment, becomes methylated, resulting in inactivation of ble-dependent resistance. 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Raeder</name></noCountry><country name="Allemagne"><noRegion><name sortKey="Gessner, M" sort="Gessner, M" uniqKey="Gessner M" first="M" last="Gessner">M. Gessner</name></noRegion></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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